Skip to Main content Skip to Navigation
Journal articles

Solubilization and stabilization of membrane proteins by cycloalkane-modified amphiphilic polymers.

Abstract : Membrane proteins need to be extracted from biological membranes and purified in their native state for most structural and functional in vitro investigations. Amphiphilic copolymers, such as amphipols (APols), have emerged as very useful alternatives to detergents for keeping membrane proteins water-soluble under their native form. However, classical APols, i.e. poly(acrylic acid) (PAA) derivatives, seldom enable direct membrane protein extraction. Poly(styrene-maleic anhydride) copolymers (SMAs), which bear aromatic rings as hydrophobic side groups, have been reported to be more effective extracting agents. In order to test the hypothesis of a role of cyclic hydrophobic moieties in membrane solubilization by copolymers, we have prepared PAA derivatives comprising cyclic rather than linear aliphatic side groups (CyclAPols). As references, APol A8-35, SMAs, and diisobutylene maleic acid (DIBMA) were compared with CyclAPols. Using as models the plasma membrane of Escherichia coli and the extraction-resistant purple membrane from Halobacterium salinarum, we show that CyclAPols combine the extraction efficiency of SMAs with the stabilization afforded to membrane proteins by classical APols such as A8-35.
Document type :
Journal articles
Complete list of metadatas

https://hal.archives-ouvertes.fr/hal-03018338
Contributor : Manuela Zoonens <>
Submitted on : Sunday, November 22, 2020 - 2:23:08 PM
Last modification on : Tuesday, December 8, 2020 - 3:35:01 AM
Long-term archiving on: : Tuesday, February 23, 2021 - 9:39:25 PM

File

Marconnet-2020.pdf
Files produced by the author(s)

Identifiers

Citation

Anaïs Marconnet, Baptiste Michon, Christel Le Bon, Fabrice Giusti, Christophe Tribet, et al.. Solubilization and stabilization of membrane proteins by cycloalkane-modified amphiphilic polymers.. Biomacromolecules, American Chemical Society, 2020, 21, pp.3459−3467. ⟨10.1021/acs.biomac.0c00929⟩. ⟨hal-03018338⟩

Share

Metrics

Record views

32

Files downloads

51