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Journal Articles iScience Year : 2022

A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment

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Chunxue Han
  • Function : Author

Abstract

Radiation therapy damages tumors and normal tissues, probably in part through the recruitment of immune cells. Endothelial high-mannose N-glycans are, in particular, involved in monocyte-endothelium interactions. Trimmed by the class I α-mannosidases, these structures are quite rare in normal conditions. Here, we show that the expression of the endothelial α-mannosidase MAN1C1 protein decreases after irradiation. We modeled two crucial steps in monocyte recruitment by developing in vitro real-time imaging models. Inhibition of MAN1C1 expression by siRNA gene silencing increases the abundance of high-mannose N-glycans, improves the adhesion of monocytes on endothelial cells in flow conditions and, in contrast, decreases radiation-induced transendothelial migration of monocytes. Consistently, overexpression of MAN1C1 in endothelial cells using lentiviral vectors decreases the abundance of high-mannose N-glycans and monocyte adhesion and enhances transendothelial migration of monocytes. Hence, we propose a role for endothelial MAN1C1 in the recruitment of monocytes, particularly in the adhesion step to the endothelium.
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Dates and versions

irsn-03888401 , version 1 (07-12-2022)

Licence

Attribution - NonCommercial - NoDerivatives - CC BY 4.0

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Ségolène Ladaigue, Anne-Charlotte Lefranc, Khadidiatou Balde, Monica Quitoco, Emilie Bacquer, et al.. A role for endothelial alpha-mannosidase MAN1C1 in radiation-induced immune cell recruitment. iScience, 2022, 25 (12), pp.105482. ⟨10.1016/j.isci.2022.105482⟩. ⟨irsn-03888401⟩
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