Graphene oxide worsens copper-mediated embryo-larval toxicity in the pacific oyster while reduced graphene oxide mitigates the effects

Antoine Mottier (1) , Morgan Légnani (2) , Frédéric Candaudap (3) , Emmanuel Flahaut (2) , Florence Mouchet (1) , Laury Gauthier (1) , Lauris Evariste (4)

1 LEFE - Laboratoire Ecologie Fonctionnelle et Environnement
2 CIRIMAT - Centre interuniversitaire de recherche et d'ingenierie des matériaux
3 GET - Géosciences Environnement Toulouse
4 ToxAlim-ENTeRisk - Endocrinologie & Toxicologie de la Barrière Intestinale

Due to their properties, graphene-based nanomaterials (GBMs) are triggering a great interest leading to an increase of their global production and use in new applications. As a consequence, their release into the environment is expected to increase in the next years. When considering the current knowledge in the evaluation of GBMs ecotoxic potential, studies aiming to evaluate the hazard associated to these nanomaterials towards marine species and particularly considering potential interactions with other environmental pollutants such as metals are scarce. Here we evaluated the embryotoxic potential of GBMs, which include graphene oxide (GO) and its reduced form (rGO), both individually and in combination with copper (Cu) as a referent toxicant, towards early life stages of the Pacific oyster through the use of a standardized method (NF ISO 17244). We found that following exposure to Cu, dose-dependent decrease in the proportion of normal larvae was recorded with an Effective Concentration leading to the occurrence of 50% of abnormal larvae (EC50) of 13.85 ± 1.21 μg/L. Interestingly, the presence of GO at a non-toxic dose of 0.1 mg/L decreased the Cu EC50 to 12.04 ± 0.85 μg/L while it increased to 15.91 ± 1.57 μg/L in presence of rGO. Based on the measurement of copper adsorption, the obtained results suggest that GO enhances Cu bioavailability, potentially modifying its toxic pathways, while rGO mitigates Cu toxicity by decreasing its bioavailability. This research underscores the need to characterize the risk associated to GBMs interactions with other aquatic contaminants and supports the adoption of a safer-by-design strategy using rGO in marine environments. This would contribute to minimize the potential adverse effects on aquatic species and to reduce the risk for economic activities associated to coastal environments.

Mots clés: Ecotoxicology; nanomaterials; combined toxicity; safer-by-design; marine environment

Domaines: Ecotoxicologie; Zoologie des invertébrés

Antoine Mottier, Morgan Légnani, Frédéric Candaudap, Emmanuel Flahaut, Florence Mouchet, et al.. Graphene oxide worsens copper-mediated embryo-larval toxicity in the pacific oyster while reduced graphene oxide mitigates the effects. Chemosphere, 2023, 335, pp.139140. ⟨10.1016/J.CHEMOSPHERE.2023.139140⟩. ⟨hal-04158264⟩